ARG81933

Human ADAM15 ELISA Kit

Human ADAM15 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81933-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81933-002 Standard 2 X 10 ng/vial 4°C
ARG81933-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81933-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81933-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81933-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81933-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81933-008 25X Wash buffer 20 ml 4°C
ARG81933-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81933-010 STOP solution 10 ml (Ready to use) 4°C
ARG81933-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81933 Human ADAM15 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ADAM15 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name ADAM15
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.5%; Inter-Assay CV: 6.6%
Alternate Names ADAM 15; MDC-15; EC 3.4.24.-; Metargidin; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15; Metalloprotease RGD disintegrin protein; MDC15; Disintegrin and metalloproteinase domain-containing protein 15

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8751 Human ADAM15

Swiss-port # Q13444 Human Disintegrin and metalloproteinase domain-containing protein 15

Gene Symbol ADAM15
Gene Full Name ADAM metallopeptidase domain 15
Background The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Function Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. [UniProt]
Highlight Related products:
ADAM antibodies; ADAM ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM The precursor is cleaved by a furin endopeptidase.

Phosphorylation increases association with PTKs. [UniProt]
Title Download Link
ARG81933 Human ADAM15 ELISA Kit User's manual download icon Download