ARG81558

Human ADAM9 ELISA Kit

Human ADAM9 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81558-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81558-002 Standard 2 X 10 ng/vial 4°C
ARG81558-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81558-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81558-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81558-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81558-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81558-008 25X Wash buffer 20 ml 4°C
ARG81558-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81558-010 STOP solution 10 ml (Ready to use) 4°C
ARG81558-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81558 Human ADAM9 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ADAM9 in serum, plasma (heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name ADAM9
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma (heparin) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.5%
Inter-Assay CV: 6.3%
Alternate Names MDC9; Meltrin-gamma; ADAM 9; Metalloprotease/disintegrin/cysteine-rich protein 9; Disintegrin and metalloproteinase domain-containing protein 9; Cellular disintegrin-related protein; EC 3.4.24.-; CORD9; Mltng; MCMP; Myeloma cell metalloproteinase

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8754 Human ADAM9

Swiss-port # Q13443 Human Disintegrin and metalloproteinase domain-containing protein 9

Gene Symbol ADAM9
Gene Full Name ADAM metallopeptidase domain 9
Background This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2010]
Function Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activity for APP. [UniProt]
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ADAM antibodies; ADAM ELISA Kits;
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PTM Proteolytically cleaved in the trans-Golgi network before it reaches the plasma membrane to generate a mature protein. The removal of the pro-domain occurs via cleavage at two different sites. Processed most likely by a pro-protein convertase such as furin, at the boundary between the pro-domain and the catalytic domain. An additional upstream cleavage pro-protein convertase site (Arg-56/Glu-57) has an important role in the activation of ADAM9.

Phosphorylation is induced in vitro by phorbol-12-myristate-13-acetate (PMA). [UniProt]
Title Download Link
ARG81558 Human ADAM9 ELISA Kit User's manual download icon Download