ARG81557

Human ADAMTS1 ELISA Kit

Human ADAMTS1 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81557-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81557-002 Standard 2 X 10 ng/vial 4°C
ARG81557-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81557-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81557-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81557-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81557-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81557-008 25X Wash buffer 20 ml 4°C
ARG81557-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81557-010 STOP solution 10 ml (Ready to use) 4°C
ARG81557-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81557 Human ADAMTS1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ADAMTS1 in serum, plasma (heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name ADAMTS1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 46.9 pg/ml
Sample Type Serum, plasma (heparin) and cell culture supernatants.
Standard Range 93.8 - 6000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.3%
Inter-Assay CV: 7.5%
Alternate Names ADAMTS-1; A disintegrin and metalloproteinase with thrombospondin motifs 1; METH-1; METH1; EC 3.4.24.-; ADAM-TS1; C3-C5; ADAM-TS 1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 9510 Human ADAMTS1

Swiss-port # Q9UHI8 Human A disintegrin and metalloproteinase with thrombospondin motifs 1

Gene Symbol ADAMTS1
Gene Full Name ADAM metallopeptidase with thrombospondin type 1 motif, 1
Background This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function. [provided by RefSeq, Jul 2008]
Function Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture. [UniProt]
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PTM The precursor is cleaved by a furin endopeptidase.

Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity). [UniProt]
Title Download Link
ARG81557 Human ADAMTS1 ELISA Kit User's manual download icon Download