ARG82250

Human CA1 / Carbonic Anhydrase 1 ELISA Kit

Human CA1 / Carbonic Anhydrase 1 ELISA Kit for ELISA and Human

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Component

Cat No Component Name Package Temp
ARG82250-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82250-002 Standard 2 X 20 ng/vial 4°C
ARG82250-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82250-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82250-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82250-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82250-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82250-008 25X Wash buffer 20 ml 4°C
ARG82250-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82250-010 STOP solution 10 ml (Ready to use) 4°C
ARG82250-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82250 Human CA1 / Carbonic Anhydrase 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CA1 / Carbonic Anhydrase 1 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name CA1 / Carbonic Anhydrase 1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.6%
Inter-Assay CV: 5.1%
Alternate Names Carbonic anhydrase I; EC 4.2.1.1; Carbonate dehydratase I; Carbonic anhydrase B; Car1; HEL-S-11; CA-I; Carbonic anhydrase 1; CAB

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 759 Human CA1

Swiss-port # P00915 Human Carbonic anhydrase 1

Gene Symbol CA1
Gene Full Name carbonic anhydrase I
Background Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Function Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. [UniProt]
Cellular Localization Cytoplasm. [UniProt]
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Carbonic Anhydrase antibodies; Carbonic Anhydrase ELISA Kits;
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Simplify the ELISA analysis by GainData

Specific References

Executive dysfunction in euthymic bipolar disorder patients and its association with plasma biomarkers

ELISA / Human / Plasma

Izabela Guimarães Barbosa et al.
J Affect Disord.,  (2021)

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Identification of potential plasma protein biomarkers for bipolar II disorder: a preliminary/exploratory study.

ELISA / Human / Plasma

Sheng-Yu Lee et al.
Sci Rep.,  (2021)

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Title Download Link
ARG82250 Human CA1 / Carbonic Anhydrase 1 ELISA Kit User manual download icon Download