ARG82250
Human CA1 / Carbonic Anhydrase 1 ELISA Kit
Human CA1 / Carbonic Anhydrase 1 ELISA Kit for ELISA and Human
Publication2
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82250-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82250-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG82250-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82250-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82250-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82250-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82250-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82250-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82250-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82250-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82250-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82250 Human CA1 / Carbonic Anhydrase 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CA1 / Carbonic Anhydrase 1 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CA1 / Carbonic Anhydrase 1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.6% Inter-Assay CV: 5.1% |
| Alternate Names | Carbonic anhydrase I; EC 4.2.1.1; Carbonate dehydratase I; Carbonic anhydrase B; Car1; HEL-S-11; CA-I; Carbonic anhydrase 1; CAB |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CA1 |
| Gene Full Name | carbonic anhydrase I |
| Background | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| Function | Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Highlight | Related products: Carbonic Anhydrase antibodies; Carbonic Anhydrase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Specific References
Executive dysfunction in euthymic bipolar disorder patients and its association with plasma biomarkers
ELISA / Human / Plasma
Identification of potential plasma protein biomarkers for bipolar II disorder: a preliminary/exploratory study.
ELISA / Human / Plasma
| Title | Download Link |
|---|---|
| ARG82250 Human CA1 / Carbonic Anhydrase 1 ELISA Kit User manual |
 Download
|
