ARG82599

Human CA14 / Carbonic Anhydrase 14 ELISA Kit

Human CA14 / Carbonic Anhydrase 14 ELISA Kit for ELISA and Human

Overview

Product Description ARG82599 Human CA14 / Carbonic Anhydrase 14 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CA14 / Carbonic Anhydrase 14 in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity This kit could assay both natural and recombinant Human CA14.

No significant cross-reactivity or interference was observed in the following samples:
Human: IFN gamma, IL1 beta, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL17A, IL18, IL21, IL22, IL23, MCP1, TGF beta 1, TNF alpha and VEGF.
Mouse: GM-CSF, IFN gamma, IL1 beta, IL2, IL4, IL6, IL10, IL17A and TNF alpha.
Rat: IFN gamma, IL1 beta, IL4, IL6, IL10 and TNF alpha.
Target Name CA14 / Carbonic Anhydrase 14
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.16 ng/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 0.31 - 20 ng/ml
Sample Volume 50 µl
Precision Intra-Assay CV: 4.0%
Inter-Assay CV: 4.2%
Alternate Names Carbonate dehydratase XIV; CA-XIV; Carbonic anhydrase 14; EC 4.2.1.1; CAXiV; Carbonic anhydrase XIV

Application Instructions

Assay Time ~ 2.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23632 Human CA14

Swiss-port # Q9ULX7 Human Carbonic anhydrase 14

Gene Symbol CA14
Gene Full Name carbonic anhydrase XIV
Background Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA XIV is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. [provided by RefSeq, Jul 2008]
Function Reversible hydration of carbon dioxide. [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. [UniProt]
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