ARG81940

Human CD328 / SIGLEC7 ELISA Kit

Human CD328 / SIGLEC7 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81940-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81940-002 Standard 2 X 10 ng/vial 4°C
ARG81940-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81940-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81940-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81940-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81940-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81940-008 25X Wash buffer 20 ml 4°C
ARG81940-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81940-010 STOP solution 10 ml (Ready to use) 4°C
ARG81940-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81940 Human CD328 / SIGLEC7 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD328 / SIGLEC7 in serum, plasma (heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD328 / Siglec 7
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 62.5 pg/ml
Sample Type Serum, plasma (heparin) and cell culture supernatants.
Standard Range 125 - 8000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.0%; Inter-Assay CV: 6.6%
Alternate Names QA79 membrane protein; p75; CD antigen CD328; SIGLEC19P; Adhesion inhibitory receptor molecule 1; SIGLECP2; Sialic acid-binding Ig-like lectin 7; SIGLEC-7; p75/AIRM1; CDw328; Siglec-7; AIRM-1; CD328; AIRM1; D-siglec; QA79

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 27036 Human SIGLEC7

Swiss-port # Q9Y286 Human Sialic acid-binding Ig-like lectin 7

Gene Symbol SIGLEC7
Gene Full Name sialic acid binding Ig-like lectin 7
Function Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro). [UniProt]
Highlight Related products:
CD328 antibodies; CD328 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Tyrosine phosphorylated. [UniProt]
Title Download Link
ARG81940 Human CD328 / SIGLEC7 ELISA Kit User's manual download icon Download