ARG82756

Human CD85j / LIR1 ELISA Kit

Human CD85j / LIR1 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82756-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82756-002 Standard 2 X 10 ng/vial 4°C
ARG82756-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82756-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82756-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82756-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82756-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82756-008 25X Wash buffer 20 ml 4°C
ARG82756-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82756-010 STOP solution 10 ml (Ready to use) 4°C
ARG82756-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82756 Human CD85j / LIR1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD85j / LIR1 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name CD85j / LIR1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.3%
Inter-Assay CV: 6.4%
Alternate Names LIR-1; Leukocyte immunoglobulin-like receptor 1; ILT2; CD85 antigen-like family member J; Immunoglobulin-like transcript 2; LIR1; ILT-2; Leukocyte immunoglobulin-like receptor subfamily B member 1; Monocyte/macrophage immunoglobulin-like receptor 7; CD antigen CD85j; CD85J; MIR7; MIR-7

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10859 Human LILRB1

Swiss-port # Q8NHL6 Human Leukocyte immunoglobulin-like receptor subfamily B member 1

Gene Symbol LILRB1
Gene Full Name leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
Background This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Isoform 5: Secreted. [UniProt]
Highlight Related products:
CD85 antibodies; CD85 ELISA Kits;
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PTM Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6. [UniProt]
Title Download Link
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