ARG81627

Human CDCP1 ELISA Kit

Human CDCP1 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81627-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81627-002 Standard 2 X 10 ng/vial 4°C
ARG81627-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81627-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81627-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81627-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81627-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81627-008 25X Wash buffer 20 ml 4°C
ARG81627-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81627-010 STOP solution 10 ml (Ready to use) 4°C
ARG81627-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81627 Human CDCP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CDCP1 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CDCP1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.4%
Inter-Assay CV: 7.0%
Alternate Names Membrane glycoprotein gp140; CD antigen CD318; SIMA135; Subtractive immunization M plus HEp3-associated 135 kDa protein; CUB domain-containing protein 1; TRASK; Transmembrane and associated with src kinases; CD318

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 64866 Human CDCP1

Swiss-port # Q9H5V8 Human CUB domain-containing protein 1

Gene Symbol CDCP1
Gene Full Name CUB domain containing protein 1
Background This gene encodes a transmembrane protein which contains three extracellular CUB domains and acts as a substrate for Src family kinases. The protein plays a role in the tyrosine phosphorylation-dependent regulation of cellular events that are involved in tumor invasion and metastasis. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]
Function May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis. [UniProt]
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CDCP1 antibodies; CDCP1 ELISA Kits;
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PTM Phosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5.

N-glycosylated.

A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease. [UniProt]
Title Download Link
ARG81627 Human CDCP1 ELISA Kit User manual download icon Download