ARG82576

Human Cadherin 15 ELISA Kit

Human Cadherin 15 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82576-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82576-002 Standard 2 X 10 ng/vial 4°C
ARG82576-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82576-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82576-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82576-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82576-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82576-008 25X Wash buffer 20 ml 4°C
ARG82576-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82576-010 STOP solution 10 ml (Ready to use) 4°C
ARG82576-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82576 Human Cadherin 15 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cadherin 15 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name Cadherin 15
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.3%
Inter-Assay CV: 5.9%
Alternate Names CDHM; CDH14; MRD3; MCAD; Cadherin-15; Cadherin-14; Muscle cadherin; M-cadherin; CDH3

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1013 Human CDH15

Swiss-port # P55291 Human Cadherin-15

Gene Symbol CDH15
Gene Full Name cadherin 15, type 1, M-cadherin (myotubule)
Background This gene is a member of the cadherin superfamily of genes, encoding calcium-dependent intercellular adhesion glycoproteins. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. The protein is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation. [provided by RefSeq, Jul 2008]
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
Highlight Related products:
Cadherin antibodies; Cadherin ELISA Kits; Cadherin Duos / Panels;
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Title Download Link
ARG82576 Human Cadherin 15 ELISA Kit User manual download icon Download