ARG81090

Human Complement C3 ELISA Kit

Human Complement C3 ELISA Kit for ELISA and Human

Signaling Transduction kit

Overview

Product Description

ARG81090 Human Complement C3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Complement C3 in plasma, serum, urine, milk, saliva and cell culture media.

Tested Reactivity Hu
Tested Application ELISA
Target Name Complement C3
Conjugation HRP
Conjugation Note

TMB substrate is used for color development at 450 nm.

Sensitivity 0.0148 ng/ml
Detection Range 0.02 - 10 ng/ml
Sample Type Plasma, serum, urine, milk, saliva and cell culture media
Alternate Names CPAMD1; HEL-S-62p; ASP; ARMD9; C3a; C3b; C3bc; Complement C3; C3adesArg; AHUS5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 718 Human C3

Swiss-port # P01024 Human Complement C3

Gene Symbol C3
Gene Full Name complement component 3
Background Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. A peptide (C3a) derived from the encoded protein has antimicrobial activity, so people with C3 deficiency are susceptible to bacterial infection. [provided by RefSeq, Nov 2014]
Function C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.

C3-beta-c: Acts as a chemoattractant for neutrophils in chronic inflammation.

Acylation stimulating protein: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2. [UniProt]
Highlight Related products:
Complement component antibodies; Complement component ELISA Kits; Complement component Duos / Panels;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Signaling Transduction kit
PTM C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. C3a is further processed by carboxypeptidases to release the C-terminal arginine residue generating the acylation stimulating protein (ASP). Levels of ASP are increased in adipocytes in the postprandial period and by insulin and dietary chylomicrons.
Phosphorylated by FAM20C in the extracellular medium.
Title Download Link
ARG81090 Human Complement C3 ELISA Kit User's manual download icon Download