ARG82647
Human Complement Factor I ELISA Kit
Human Complement Factor I ELISA Kit for ELISA and Human
Overview
| Product Description | ARG82647 Human Complement Factor I ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Complement Factor I in serum, plasma, cell culture supernatants, saliva and milk. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Cross-Reactivity: Pig: 75% Rat: 5% Mouse, Monkey, Bovine, Dog and Rabbit: None No significant cross-reactivity observed with complement factor B, factor D, factor H, factor P, C1, C2, C3, C4, C5, C6, C7, C8 and C9. |
| Target Name | Complement Factor I |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.28 µg/ml |
| Sample Type | Serum, plasma, cell culture supernatants, saliva and milk. |
| Standard Range | 0.375 - 24 µg/ml |
| Sample Volume | 25 µl |
| Precision | Intra-Assay CV: 5.4% Inter-Assay CV: 9.8% |
| Alternate Names | Complement factor I; KAF; C3B/C4B inactivator; C3b-INA; ARMD13; EC 3.4.21.45; FI; AHUS3; C3BINA; IF |
Application Instructions
| Assay Time | ~ 3 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | CFI |
| Gene Full Name | complement factor I |
| Background | This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uremic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immune deposits and age-related macular degeneration are other conditions associated with mutations of this gene. [provided by RefSeq, Dec 2015] |
| Function | Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins (PubMed:7360115, PubMed:17320177). Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces (PubMed:2141838, PubMed:9605165, PubMed:12055245). The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization (PubMed:28671664). [UniProt] |
| Cellular Localization | Secreted, extracellular space. [UniProt] |
| Highlight | Related products: Complement component antibodies; Complement component ELISA Kits; Complement component Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
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| ARG82647 Human Complement Factor I ELISA kit User manual |
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