ARG80185

Human GRO beta / CXCL2 ELISA Kit

Human GRO beta / CXCL2 ELISA Kit for ELISA and Human

Immune System kit
publication_link Publication2

Component

Cat No Component Name Package Temp
ARG80185-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80185-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80185-003 Standard diluent buffer 20 ml 4°C
ARG80185-004 Antibody conjugate concentrate 400 µl 4°C
ARG80185-005 Antibody diluent buffer 16 ml 4°C
ARG80185-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80185-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80185-008 20X Wash buffer 50 ml 4°C
ARG80185-009 TMB substrate 12ml 4°C (Protect from light)
ARG80185-010 STOP solution 12ml 4°C
ARG80185-011 Plate sealer 4 strips Room temperature

Overview

Product Description

ARG80185 Human GRO beta / CXCL2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human GRO beta / CXCL2 in Serum, Plasma, Cell culture supernatants.

Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human CXCL1, CXCL3, CXCL4, IL-2,3,4,5,6,7,9,10,12, IFN gamma and TNF alpha.
Target Name CXCL2 / MIP2
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 0.8 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 1.56 - 100 pg/ml
Sample Volume 100 µl
Precision

Intra-Assay CV: <10%; Inter-Assay CV: <10%

Alternate Names Gro-beta; SCYB2; HSF; CINC-2a; GROb; MGSA-b; SB-251353; MIP2A; MIP2; Hematopoietic synergistic factor; 5-73; C-X-C motif chemokine 2; MIP-2a; GRO2; Macrophage inflammatory protein 2-alpha; GRO-beta-T; Growth-regulated protein beta; MIP2-alpha

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2920 Human CXCL2

Swiss-port # P19875 Human C-X-C motif chemokine 2

Gene Symbol CXCL2
Gene Full Name chemokine (C-X-C motif) ligand 2
Background Chemokine (C-X-C motif) ligand 2 (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2). CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1. This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. The gene for CXCL2 is located on human chromosome 4 in a cluster of other CXC chemokines. CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2.
Highlight Related products:
GRO beta antibodies; GRO beta ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Immune System kit
PTM The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells.

Specific References

Tumor cells educate mesenchymal stromal cells to release chemoprotective and immunomodulatory factors.

ELISA / Human / Cell culture supernatant

Augustin Le Naour et al.
J Mol Cell Biol.,  (2020)

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Mesenchymal stromal cells release CXCL1/2/8 and induce chemoresistance and macrophage polarization.

ELISA / Human

Augustin Le Naour et al.
Preprint.,  (2018)

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Title Download Link
ARG80185 Human GRO beta / CXCL2 ELISA Kit User's manual download icon Download