ARG82623

Human MCP3 ELISA Kit

Human MCP3 ELISA Kit for ELISA and Human

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Component

Cat No Component Name Package Temp
ARG82623-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82623-002 Standard 2 X 10 ng/vial 4°C
ARG82623-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82623-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82623-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82623-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82623-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82623-008 25X Wash buffer 20 ml 4°C
ARG82623-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82623-010 STOP solution 10 ml (Ready to use) 4°C
ARG82623-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82623 Human MCP3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MCP3 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name MCP3
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 7.0%
Inter-Assay CV: 7.1%
Alternate Names MCP3; NC28; Monocyte chemotactic protein 3; MARC; Small-inducible cytokine A7; FIC; C-C motif chemokine 7; Monocyte chemoattractant protein 3; MCP-3; SCYA7; SCYA6

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6354 Human CCL7

Swiss-port # P80098 Human C-C motif chemokine 7

Gene Symbol CCL7
Gene Full Name chemokine (C-C motif) ligand 7
Background This gene encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q. [provided by RefSeq, Jul 2008]
Function Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. [UniProt]
Cellular Localization Secreted. [UniProt]
Highlight Related products:
MCP3 antibodies; MCP3 ELISA Kits; MCP3 Duos / Panels;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM O-glycosylated. [UniProt]

Specific References

The Synergistic Inhibition of Coronavirus Replication and Induced Cytokine Production by Ciclesonide and the Tylophorine-Based Compound Dbq33b

ELISA / Human / Culture supernatant

Yue-Zhi Lee et al.
Pharmaceutics.,  (2022)

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