ARG82693

Human MICA ELISA Kit

Human MICA ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82693-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82693-002 Standard 2 X 10 ng/vial 4°C
ARG82693-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82693-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82693-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82693-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82693-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82693-008 25X Wash buffer 20 ml 4°C
ARG82693-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82693-010 STOP solution 10 ml (Ready to use) 4°C
ARG82693-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82693 Human MICA ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MICA in serum and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name MICA
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 32 pg/ml
Sample Type Serum and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 7.3%
Inter-Assay CV: 8.1%
Alternate Names MHC class I polypeptide-related sequence A; PERB11.1; MIC-A

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 100507436 Human MICA

Swiss-port # Q29983 Human MHC class I polypeptide-related sequence A

Gene Symbol MICA
Gene Full Name MHC class I polypeptide-related sequence A
Background This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Function Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Cytoplasm. Note=Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum. [UniProt]
Highlight Related products:
MICA antibodies; MICA ELISA Kits;
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PTM N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation.

Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation. [UniProt]
Title Download Link
ARG82693 Human MICA ELISA Kit User manual download icon Download