ARG80145

Human MMP1 (Total) ELISA kit

Human MMP1 (Total) ELISA kit for ELISA and Human

Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit
publication_link Publication1

Component

Cat No Component Name Package Temp
ARG80145-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80145-002 Standard (Lyophilized) 3 X 2 ng/vial 4°C
ARG80145-003 Standard diluent buffer 20 ml 4°C
ARG80145-004 Antibody conjugate concentrate 400 µl 4°C
ARG80145-005 Antibody diluent buffer 16 ml 4°C
ARG80145-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80145-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80145-008 20X Wash buffer 50 ml 4°C
ARG80145-009 TMB substrate 12ml 4°C (Protect from light)
ARG80145-010 STOP solution 12ml 4°C
ARG80145-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80145 Human MMP1 (Total) ELISA kit is an Enzyme Immunoassay kit for the quantification of Human MMP1 (Total) in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human MMP-2,3,7,8,9,10,12,13,16, TIMP-1,2,3,4
Target Name MMP1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 30 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision CV: <10%
Alternate Names MMP-1; CLG; Fibroblast collagenase; Matrix metalloproteinase-1; CLGN; EC 3.4.24.7; Interstitial collagenase

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4312 Human MMP1

Swiss-port # P03956 Human Interstitial collagenase

Gene Symbol MMP1
Gene Full Name matrix metallopeptidase 1
Background The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. In addition, fibronectin-like repeats, a hinge region, and a C-terminal hemopexin-like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.
Highlight Related products:
MMP1 antibodies; MMP1 ELISA Kits;
Related news:
Detecting MMPs and their non-ECM substrates
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit
PTM Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
Tyrosine phosphorylated in platelets by PKDCC/VLK.

Specific References

Adipose-derived stem cells enriched with therapeutic mRNA TGF-β3 and IL-10 synergistically promote scar-less wound healing in preclinical models

ELISA / Human / Culture supernatant

Wei Wang et al.
Bioengineering & Translational Medicine,  (2023)

publication_link

 

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