ARG80145
Human MMP1 (Total) ELISA kit
Human MMP1 (Total) ELISA kit for ELISA and Human
Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit
Publication1
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80145-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80145-002 | Standard (Lyophilized) | 3 X 2 ng/vial | 4°C |
| ARG80145-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80145-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80145-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80145-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80145-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80145-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80145-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80145-010 | STOP solution | 12ml | 4°C |
| ARG80145-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80145 Human MMP1 (Total) ELISA kit is an Enzyme Immunoassay kit for the quantification of Human MMP1 (Total) in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human MMP-2,3,7,8,9,10,12,13,16, TIMP-1,2,3,4 |
| Target Name | MMP1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 30 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | MMP-1; CLG; Fibroblast collagenase; Matrix metalloproteinase-1; CLGN; EC 3.4.24.7; Interstitial collagenase |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MMP1 |
| Gene Full Name | matrix metallopeptidase 1 |
| Background | The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. In addition, fibronectin-like repeats, a hinge region, and a C-terminal hemopexin-like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis. |
| Highlight | Related products: MMP1 antibodies; MMP1 ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit |
| PTM | Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. Tyrosine phosphorylated in platelets by PKDCC/VLK. |
Specific References
Adipose-derived stem cells enriched with therapeutic mRNA TGF-β3 and IL-10 synergistically promote scar-less wound healing in preclinical models
ELISA / Human / Culture supernatant
| Title | Download Link |
|---|---|
| ARG80145 Human MMP1 (Total) ELISA kit User's manual |
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