ARG70298

Human PVRL3 recombinant protein (ECD) (Fc-His-tagged, C-ter)

Human PVRL3 recombinant protein (ECD) (Fc-His-tagged, C-ter) for SDS-PAGE,Binding Activity and Human

Overview

Product Description HEK293 expressed, Fc-His-tagged (C-ter) Human PVRL3 recombinant protein (ECD).
Tested Reactivity Hu
Tested Application Binding, SDS-PAGE
Target Name PVRL3 (ECD)
Species Human
A.A. Sequence Gly58 - Asp400 of Human PVRL3 (NP_056295) with an Fc-6X His tag at the C-terminus.
Expression System HEK293
Alternate Names PVRR3; PPR3; CDW113; PRR3; Nectin-3; CDw113; CD113; NECTIN-3; Poliovirus receptor-related protein 3; CD antigen CD113

Application Instructions

Application Note Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Nectin-1 at 3µg/ml (100 µl/well) can bind Recombinant Human Nectin-3 with a linear range of 180-720 ng/ml.

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 97% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25945 Human PVRL3

Swiss-port # Q9NQS3 Human Nectin-3

Gene Symbol PVRL3
Gene Full Name poliovirus receptor-related 3
Background This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
Function Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body. [UniProt]
Cellular Localization Cell membrane; Single-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane. [UniProt]
Calculated MW 61 kDa