ARG81526

Human SPARC ELISA Kit

Human SPARC ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81526-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81526-002 Standard 2 X 50 ng/vial 4°C
ARG81526-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81526-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81526-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81526-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81526-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81526-008 25X Wash buffer 20 ml 4°C
ARG81526-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81526-010 STOP solution 10 ml (Ready to use) 4°C
ARG81526-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81526 Human SPARC ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human SPARC in serum, human milk and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name SPARC
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.39 ng/ml
Sample Type Serum, human milk and cell culture supernatants.
Standard Range 0.78 - 50 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.5%
Inter-Assay CV: 7.5%
Alternate Names ON; BM-40; Basement-membrane protein 40; SPARC; Osteonectin; Secreted protein acidic and rich in cysteine

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6678 Human SPARC

Swiss-port # P09486 Human SPARC

Gene Symbol SPARC
Gene Full Name secreted protein, acidic, cysteine-rich (osteonectin)
Background This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]
Function Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. [UniProt]
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Title Download Link
ARG81526 Human SPARC ELISA Kit User's manual download icon Download