ARG81453

Human TNFRSF18 ELISA Kit

Human TNFRSF18 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81453-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81453-002 Standard 2 X 10 ng/vial 4°C
ARG81453-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81453-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81453-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81453-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81453-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81453-008 25X Wash buffer 20 ml 4°C
ARG81453-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81453-010 STOP solution 10 ml (Ready to use) 4°C
ARG81453-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81453 Human TNFRSF18 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TNFRSF18 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name TNFRSF18
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.3%
Inter-Assay CV: 7.0%
Alternate Names Tumor necrosis factor receptor superfamily member 18; AITR; CD357; CD antigen CD357; Activation-inducible TNFR family receptor; GITR-D; GITR; Glucocorticoid-induced TNFR-related protein

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8784 Human TNFRSF18

Swiss-port # Q9Y5U5 Human Tumor necrosis factor receptor superfamily member 18

Gene Symbol TNFRSF18
Gene Full Name tumor necrosis factor receptor superfamily, member 18
Background This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Feb 2011]
Function Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway. [UniProt]
Highlight Related products:
TNFRSF antibodies; TNFRSF ELISA Kits;
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Title Download Link
ARG81453 Human TNFRSF18 ELISA Kit User's manual download icon Download