ARG81430

Human VEGFC ELISA Kit

Human VEGFC ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81430-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81430-002 Standard 2 X 10 ng/vial 4°C
ARG81430-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81430-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81430-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81430-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81430-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81430-008 25X Wash buffer 20 ml 4°C
ARG81430-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81430-010 STOP solution 10 ml (Ready to use) 4°C
ARG81430-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81430 Human VEGFC ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human VEGFC in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name VEGFC
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.6%
Inter-Assay CV: 6.8%
Alternate Names VRP; Flt4-L; VEGF-C; Vascular endothelial growth factor-related protein; Flt4 ligand; Vascular endothelial growth factor C; LMPH1D

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7424 Human VEGFC

Swiss-port # P49767 Human Vascular endothelial growth factor C

Gene Symbol VEGFC
Gene Full Name vascular endothelial growth factor C
Background The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. The encoded protein promotes angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. The proprotein is further cleaved into a fully processed form that can bind and activate VEGFR-2 and VEGFR-3 receptors. [provided by RefSeq, Apr 2014]
Function Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. [UniProt]
Highlight Related products:
VEGF antibodies; VEGF ELISA Kits; VEGF Duos / Panels; VEGF recombinant proteins;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-227 and Ser-228 producing a heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions. [UniProt]
Title Download Link
ARG81430 Human VEGFC ELISA Kit User's manual download icon Download