ARG80183

Human sRANK Ligand / TNFSF11 / TRANCE ELISA Kit

Human sRANK Ligand / TNFSF11 / TRANCE ELISA Kit for ELISA and Human

Cell Biology and Cellular Response kit; Immune System kit
publication_link Publication1

Component

Cat No Component Name Package Temp
ARG80183-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80183-002 Standard (Lyophilized) 3 X 2 ng/vial 4°C
ARG80183-003 Standard diluent buffer 20 ml 4°C
ARG80183-004 Antibody conjugate concentrate 400 µl 4°C
ARG80183-005 Antibody diluent buffer 16 ml 4°C
ARG80183-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80183-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80183-008 20X Wash buffer 50 ml 4°C
ARG80183-009 TMB substrate 12ml 4°C (Protect from light)
ARG80183-010 STOP solution 12ml 4°C
ARG80183-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80183 Human sRANK Ligand / TNFSF11 / TRANCE ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human sRANK Ligand / TNFSF11 / TRANCE in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IL-2,3,4,5,6,7,9,10,12, IFN- gamma, TNF- alpha
Target Name sRANK Ligand / TNFSF11 / TRANCE
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 8 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names TRANCE; Osteoprotegerin ligand; CD254; sOdf; Receptor activator of nuclear factor kappa-B ligand; OPTB2; RANKL; OPGL; Tumor necrosis factor ligand superfamily member 11; hRANKL2; TNF-related activation-induced cytokine; Osteoclast differentiation factor; ODF; CD antigen CD254

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8600 Human TNFSF11

Swiss-port # O14788 Human Tumor necrosis factor ligand superfamily member 11

Gene Symbol TNFSF11
Gene Full Name tumor necrosis factor (ligand) superfamily, member 11
Background This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced tra
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Research Area Cell Biology and Cellular Response kit; Immune System kit
PTM The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.

Specific References

sTNFRII-Fc modification protects human UC-MSCs against apoptosis/autophagy induced by TNF-α and enhances their efficacy in alleviating inflammatory arthritis.

ELISA / Human / Cell culture medium

Yingjie Zhao et al.
Preprint.,  (2021)

publication_link

 

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