ARG81679

Mouse ACE ELISA Kit

Mouse ACE ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81679-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81679-002 Standard 2 X 10 ng/vial 4°C
ARG81679-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81679-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81679-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81679-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81679-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81679-008 25X Wash buffer 20 ml 4°C
ARG81679-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81679-010 STOP solution 10 ml (Ready to use) 4°C
ARG81679-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81679 Mouse ACE ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse ACE in serum, plasma (heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name ACE
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 46.9 pg/ml
Sample Type Serum, plasma (heparin) and cell culture supernatants.
Standard Range 93.8 - 6000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.9%; Inter-Assay CV: 6.6%
Alternate Names DCP1; ICH; ACE; EC 3.2.1.-; MVCD3; Angiotensin-converting enzyme; Dipeptidyl carboxypeptidase I; CD143; CD antigen CD143; EC 3.4.15.1; Kininase II; ACE1; DCP

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11421 Mouse ACE

Swiss-port # P09470 Mouse Angiotensin-converting enzyme

Gene Symbol ACE
Gene Full Name angiotensin I converting enzyme
Background This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, respectively, that are equally active. [provided by RefSeq, May 2010]
Function Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. [UniProt]
Highlight Related products:
ACE antibodies; ACE ELISA Kits; ACE Duos / Panels; ACE recombinant proteins;
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PTM Phosphorylated by CK2 on Ser-1299; which allows membrane retention. [UniProt]
Title Download Link
ARG81679 Mouse ACE ELISA Kit User's manual download icon Download