ARG81839

Mouse ACE2 ELISA Kit

Mouse ACE2 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81839-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81839-002 Standard 2 X 10 ng/vial 4°C
ARG81839-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81839-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81839-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81839-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81839-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81839-008 25X Wash buffer 20 ml 4°C
ARG81839-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81839-010 STOP solution 10 ml (Ready to use) 4°C
ARG81839-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81839 Mouse ACE2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse ACE2 in serum, plasma (heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name ACE2
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.2%; Inter-Assay CV: 6.9%
Alternate Names Angiotensin-converting enzyme homolog; ACEH; Angiotensin-converting enzyme 2; Metalloprotease MPROT15; ACE-related carboxypeptidase; EC 3.4.17.23

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 70008 Mouse ACE2

Swiss-port # Q8R0I0 Mouse Angiotensin-converting enzyme 2

Gene Symbol ACE2
Gene Full Name angiotensin I converting enzyme 2
Background The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]
Function Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. [UniProt]
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Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1. [UniProt]
Title Download Link
ARG81839 Mouse ACE2 ELISA Kit User's manual download icon Download