ARG82280

Mouse Apolipoprotein E ELISA Kit

Mouse Apolipoprotein E ELISA Kit for ELISA and Mouse

Overview

Product Description ARG82280 Mouse Apolipoprotein E ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Apolipoprotein E in serum, plasma and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name Apolipoprotein E
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 1 ng/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 1.56 - 100 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 3.7%
Inter-Assay CV: 7.1%
Alternate Names Apolipoprotein E; Apo-E; APO-E; LPG; AD2; LDLCQ5

Application Instructions

Assay Time ~ 4.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11816 Mouse APOE

Swiss-port # P08226 Mouse Apolipoprotein E

Gene Symbol APOE
Gene Full Name apolipoprotein E
Background The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
Function Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. [UniProt]
Cellular Localization Secreted. [UniProt]
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Apolipoprotein antibodies; Apolipoprotein ELISA Kits;
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Simplify the ELISA analysis by GainData
PTM Synthesized with the sialic acid attached by O-glycosidic linkage and is subsequently desialylated in plasma. O-glycosylated with core 1 or possibly core 8 glycans. Thr-307 and Ser-314 are minor glycosylation sites compared to Ser-308.

Glycated in plasma VLDL of normal subjects, and of hyperglycemic diabetic patients at a higher level (2-3 fold).

Phosphorylated by FAM20C in the extracellular medium. [UniProt]
Title Download Link
ARG82280 Mouse Apolipoprotein E ELISA Kit User's manual download icon Download