ARG82735

Mouse CA12 / Carbonic Anhydrase 12 ELISA Kit

Mouse CA12 / Carbonic Anhydrase 12 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82735-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82735-002 Standard 2 X 10 ng/vial 4°C
ARG82735-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82735-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82735-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82735-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82735-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82735-008 25X Wash buffer 20 ml 4°C
ARG82735-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82735-010 STOP solution 10 ml (Ready to use) 4°C
ARG82735-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82735 Mouse CA12 / Carbonic Anhydrase 12 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CA12 / Carbonic Anhydrase 12 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name CA12 / Carbonic Anhydrase 12
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 10 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.9%
Inter-Assay CV: 6.1%
Alternate Names Carbonic anhydrase 12; EC 4.2.1.1; Carbonate dehydratase XII; Ca12; Carbonic anhydrase XII; CA-XII; AI314958; 2310047E01Rik

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol Car12
Gene Full Name carbonic anyhydrase 12
Background This gene encodes a membrane-bound member of the alpha carbonic anhydrase family of enzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. These proteins participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
Function Reversible hydration of carbon dioxide. [UniProt]
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Title Download Link
ARG82735 Mouse CA12 / Carbonic Anhydrase 12 ELISA kit User manual download icon Download