ARG82425

Mouse CA4 / Carbonic Anhydrase 4 ELISA Kit

Mouse CA4 / Carbonic Anhydrase 4 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82425-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82425-002 Standard 2 X 10 ng/vial 4°C
ARG82425-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82425-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82425-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82425-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82425-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82425-008 25X Wash buffer 20 ml 4°C
ARG82425-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82425-010 STOP solution 10 ml (Ready to use) 4°C
ARG82425-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82425 Mouse CA4 / Carbonic Anhydrase 4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CA4 / Carbonic Anhydrase 4 in serum, plasma (EDTA, heparin, citrate), cell culture supernatants and urine.
Tested Reactivity Ms
Tested Application ELISA
Target Name CA4 / Carbonic Anhydrase 4
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 50 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate), cell culture supernatants and urine.
Standard Range 78 - 5000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.4%
Inter-Assay CV: 5.9%
Alternate Names CAIV; EC 4.2.1.1; Carbonic anhydrase IV; Car4; CA-IV; Carbonate dehydratase IV; RP17; Carbonic anhydrase 4

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CA4
Gene Full Name carbonic anhydrase IV
Background Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport. [provided by RefSeq, Jul 2008]
Function Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. [UniProt]
Cellular Localization Cell membrane; Lipid-anchor, GPI-anchor. [UniProt]
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Title Download Link
ARG82425 Mouse CA4 / Carbonic Anhydrase 4 ELISA Kit User manual download icon Download