ARG81976

Mouse CA9 / Carbonic Anhydrase 9 ELISA Kit

Mouse CA9 / Carbonic Anhydrase 9 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81976-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81976-002 Standard 2 X 10 ng/vial 4°C
ARG81976-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81976-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81976-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81976-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81976-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81976-008 25X Wash buffer 20 ml 4°C
ARG81976-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81976-010 STOP solution 10 ml (Ready to use) 4°C
ARG81976-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81976 Mouse CA9 / Carbonic Anhydrase 9 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CA9 / Carbonic Anhydrase 9 in serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CA9 / Carbonic Anhydrase 9
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.8%; Inter-Assay CV: 7.6%
Alternate Names EC 4.2.1.1; MN; CAIX; pMW1; Carbonic anhydrase 9; Carbonic anhydrase IX; RCC-associated antigen G250; Membrane antigen MN; P54/58N; Carbonate dehydratase IX; Renal cell carcinoma-associated antigen G250; CA-IX

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CA9
Gene Full Name carbonic anhydrase IX
Background Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]
Function Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. [UniProt]
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Carbonic Anhydrase antibodies; Carbonic Anhydrase ELISA Kits;
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Simplify the ELISA analysis by GainData
PTM Asn-346 bears high-mannose type glycan structures. [UniProt]
Title Download Link
ARG81976 Mouse CA9 / Carbonic Anhydrase 9 ELISA Kit User's manual download icon Download