ARG82328

Mouse CD152 / CTLA4 ELISA Kit

Mouse CD152 / CTLA4 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82328-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82328-002 Standard 2 X 10 ng/vial 4°C
ARG82328-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82328-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82328-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82328-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82328-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82328-008 25X Wash buffer 20 ml 4°C
ARG82328-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82328-010 STOP solution 10 ml (Ready to use) 4°C
ARG82328-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82328 Mouse CD152 / CTLA4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD152 / CTLA4 in serum, plasma (heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name CD152 / CTLA4
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.0%
Inter-Assay CV: 5.8%
Alternate Names GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12477 Mouse CTLA4

Swiss-port # P09793 Mouse Cytotoxic T-lymphocyte protein 4

Gene Symbol CTLA4
Gene Full Name cytotoxic T-lymphocyte-associated protein 4
Background This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]
Function Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation;. [UniProt]
Highlight Related products:
CD152 antibodies; CD152 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM N-glycosylation is important for dimerization.

Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. [UniProt]
Title Download Link
ARG82328 Mouse CD152 / CTLA4 ELISA Kit User manual download icon Download