ARG82691

Mouse CD153 / CD30 Ligand ELISA Kit

Mouse CD153 / CD30 Ligand ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82691-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82691-002 Standard 2 X 10 ng/vial 4°C
ARG82691-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82691-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82691-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82691-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82691-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82691-008 25X Wash buffer 20 ml 4°C
ARG82691-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82691-010 STOP solution 10 ml (Ready to use) 4°C
ARG82691-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82691 Mouse CD153 / CD30 Ligand ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD153 / CD30 Ligand in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name CD153 / CD30 Ligand
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 8 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.9%
Inter-Assay CV: 7.9%
Alternate Names Tumor necrosis factor ligand superfamily member 8; CD30L; CD30-L; CD153; CD antigen CD153; CD30LG; CD30 ligand

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21949 Mouse TNFSF8

Swiss-port # P32972 Mouse Tumor necrosis factor ligand superfamily member 8

Gene Symbol TNFSF8
Gene Full Name tumor necrosis factor (ligand) superfamily, member 8
Background The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Function Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. [UniProt]
Cellular Localization Membrane; Single-pass type II membrane protein. [UniProt]
Highlight Related products:
CD153 antibodies; CD153 ELISA Kits;
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Title Download Link
ARG82691 Mouse CD153 / CD30 Ligand ELISA kit User manual download icon Download