ARG82311

Mouse CD22 ELISA Kit

Mouse CD22 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82311-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82311-002 Standard 2 X 10 ng/vial 4°C
ARG82311-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82311-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82311-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82311-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82311-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82311-008 25X Wash buffer 20 ml 4°C
ARG82311-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82311-010 STOP solution 10 ml (Ready to use) 4°C
ARG82311-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82311 Mouse CD22 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD22 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD22
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.078 ng/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 0.156 - 10 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.8%
Inter-Assay CV: 6.7%
Alternate Names B-lymphocyte cell adhesion molecule; B-cell receptor CD22; T-cell surface antigen Leu-14; BL-CAM; SIGLEC-2; Sialic acid-binding Ig-like lectin 2; Siglec-2; CD antigen CD22; SIGLEC2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12483 Mouse CD22

Swiss-port # P35329 Mouse B-cell receptor CD22

Gene Symbol CD22
Gene Full Name CD22 molecule
Function Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
Highlight Related products:
CD22 antibodies; CD22 ELISA Kits; CD22 Duos / Panels;
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PTM Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.

Phosphorylated on tyrosine residues by LYN. [UniProt]
Title Download Link
ARG82311 Mouse CD22 ELISA kit-User manual download icon Download