ARG82089

Mouse CD32 ELISA Kit

Mouse CD32 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82089-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82089-002 Standard 2 X 10 ng/vial 4°C
ARG82089-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82089-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82089-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82089-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82089-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82089-008 25X Wash buffer 20 ml 4°C
ARG82089-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82089-010 STOP solution 10 ml (Ready to use) 4°C
ARG82089-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82089 Mouse CD32 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD32 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD32
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.9%; Inter-Assay CV: 7.1%
Alternate Names Fc-gamma-RIIc; Fc-gamma RII-c; CD32; CD antigen CD32; CD32B; IgG Fc receptor II-c; Low affinity immunoglobulin gamma Fc region receptor II-c; FCG2; IGFR2; FcRII-c; FCGR2; CDw32

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14130 Mouse FCGR2B

Swiss-port # P08101 Mouse Low affinity immunoglobulin gamma Fc region receptor II

Gene Symbol FCGR2B
Gene Full Name Fc fragment of IgG, low affinity IIb, receptor (CD32)
Background The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Function Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
Highlight Related products:
CD32 antibodies; CD32 ELISA Kits; CD32 Duos / Panels; CD32 recombinant proteins;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Phosphorylated by the SRC-type Tyr-kinases LYN and BLK. [UniProt]
Title Download Link
ARG82089 Mouse CD32 ELISA Kit User's manual download icon Download