ARG82416

Mouse CLEC7A ELISA Kit

Mouse CLEC7A ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82416-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82416-002 Standard 2 X 10 ng/vial 4°C
ARG82416-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82416-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82416-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82416-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82416-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82416-008 25X Wash buffer 20 ml 4°C
ARG82416-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82416-010 STOP solution 10 ml (Ready to use) 4°C
ARG82416-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82416 Mouse CLEC7A ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CLEC7A in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name CLEC7A
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 50 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.4%
Inter-Assay CV: 6.0%
Alternate Names C-type lectin superfamily member 12; CD369; Dectin-1; Dendritic cell-associated C-type lectin 1; CLECSF12; CANDF4; BGR; Beta-glucan receptor; C-type lectin domain family 7 member A; DC-associated C-type lectin 1; DECTIN1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 56644 Mouse CLEC7A

Swiss-port # Q6QLQ4 Mouse C-type lectin domain family 7 member A

Gene Symbol CLEC7A
Gene Full Name C-type lectin domain family 7, member A
Background This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]
Function Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation (By similarity). [UniProt]
Cellular Localization Cell membrane; Single-pass type II membrane protein. Isoform 5: Cytoplasm. Isoform 6: Cytoplasm. Isoform 7: Cytoplasm. [UniProt]
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PTM Phosphorylated on tyrosine residues in response to glucan binding. [UniProt]
Title Download Link
ARG82416 Mouse CLEC7A ELISA Kit User manual download icon Download