ARG82416
Mouse CLEC7A ELISA Kit
Mouse CLEC7A ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82416-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82416-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82416-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82416-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82416-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82416-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82416-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82416-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82416-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82416-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82416-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82416 Mouse CLEC7A ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CLEC7A in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | CLEC7A |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 50 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.4% Inter-Assay CV: 6.0% |
| Alternate Names | C-type lectin superfamily member 12; CD369; Dectin-1; Dendritic cell-associated C-type lectin 1; CLECSF12; CANDF4; BGR; Beta-glucan receptor; C-type lectin domain family 7 member A; DC-associated C-type lectin 1; DECTIN1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q6QLQ4 Mouse C-type lectin domain family 7 member A |
|---|---|
| Gene Symbol | CLEC7A |
| Gene Full Name | C-type lectin domain family 7, member A |
| Background | This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008] |
| Function | Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation (By similarity). [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. Isoform 5: Cytoplasm. Isoform 6: Cytoplasm. Isoform 7: Cytoplasm. [UniProt] |
| Highlight | Related products: CLEC antibodies; CLEC ELISA Kits; CLEC Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated on tyrosine residues in response to glucan binding. [UniProt] |
| Title | Download Link |
|---|---|
| ARG82416 Mouse CLEC7A ELISA Kit User manual |
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