ARG82104

Mouse EFEMP1 ELISA Kit

Mouse EFEMP1 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82104-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82104-002 Standard 2 X 20 ng/vial 4°C
ARG82104-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82104-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82104-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82104-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82104-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82104-008 25X Wash buffer 20 ml 4°C
ARG82104-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82104-010 STOP solution 10 ml (Ready to use) 4°C
ARG82104-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82104 Mouse EFEMP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse EFEMP1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name EFEMP1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.156 ng/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 0.312 - 20 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.4%; Inter-Assay CV: 6.9%
Alternate Names FIBL-3; MTLV; S1-5; Fibrillin-like protein; DRAD; FBNL; Extracellular protein S1-5; Fibulin-3; MLVT; FBLN3; DHRD; EGF-containing fibulin-like extracellular matrix protein 1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 216616 Mouse EFEMP1

Swiss-port # Q8BPB5 Mouse EGF-containing fibulin-like extracellular matrix protein 1

Gene Symbol EFEMP1
Gene Full Name EGF containing fibulin-like extracellular matrix protein 1
Background This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]
Function Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth. [UniProt]
Cellular Localization Secreted, extracellular space. Secreted, extracellular space, extracellular matrix. Note=Localizes to the lamina propria underneath the olfactory epithelium. [UniProt]
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Title Download Link
ARG82104 Mouse EFEMP1 ELISA Kit User's manual download icon Download