ARG82731

Mouse ERAP1 ELISA Kit

Mouse ERAP1 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82731-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82731-002 Standard 2 X 10 ng/vial 4°C
ARG82731-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82731-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82731-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82731-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82731-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82731-008 25X Wash buffer 20 ml 4°C
ARG82731-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82731-010 STOP solution 10 ml (Ready to use) 4°C
ARG82731-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82731 Mouse ERAP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse ERAP1 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name ERAP1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.5%
Inter-Assay CV: 6.8%
Alternate Names Puromycin-insensitive leucyl-specific aminopeptidase; PILS-AP; EC 3.4.11.-; ARTS1; Adipocyte-derived leucine aminopeptidase; ERAAP1; ARTS-1; A-LAP; PILSAP; ALAP; Endoplasmic reticulum aminopeptidase 1; Aminopeptidase PILS; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator; APPILS; ERAAP

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 80898 Mouse ERAP1

Swiss-port # Q9EQH2 Mouse Endoplasmic reticulum aminopeptidase 1

Gene Symbol ERAP1
Gene Full Name endoplasmic reticulum aminopeptidase 1
Background The protein encoded by this gene is an aminopeptidase involved in trimming HLA class I-binding precursors so that they can be presented on MHC class I molecules. The encoded protein acts as a monomer or as a heterodimer with ERAP2. This protein may also be involved in blood pressure regulation by inactivation of angiotensin II. Three transcript variants encoding two different isoforms have been found for this gene.[provided by RefSeq, Oct 2010]
Function Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney. [UniProt]
Cellular Localization Endoplasmic reticulum membrane; Single-pass type II membrane protein. [UniProt]
Highlight Related products:
ERAP1 antibodies; ERAP1 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM N-glycosylated. [UniProt]
Title Download Link
ARG82731 Mouse ERAP1 ELISA kit User manual download icon Download
ARG82731 Mouse ERAP1 ELISA Kit User manual download icon Download