ARG81643

Mouse FAS Ligand ELISA Kit

Mouse FAS Ligand ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81643-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81643-002 Standard 2 X 10 ng/vial 4°C
ARG81643-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81643-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81643-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81643-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81643-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81643-008 25X Wash buffer 20 ml 4°C
ARG81643-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81643-010 STOP solution 10 ml (Ready to use) 4°C
ARG81643-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81643 Mouse FAS Ligand ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse FAS Ligand in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD178 / Fas Ligand
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.8%; Inter-Assay CV: 6.7%
Alternate Names FasL ICD; SPPL2A-processed FasL form; Apoptosis antigen ligand; CD95 ligand; CD178; Fas antigen ligand; CD95-L; Receptor-binding FasL ectodomain; FasL; SPA; TNFSF6; CD95L; FASL; Fas ligand; APTL; APT1LG1; ALPS1B; sFasL; Soluble Fas ligand; Tumor necrosis factor ligand superfamily member 6; APL; CD antigen CD178

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol FASLG
Gene Full Name Fas ligand (TNF superfamily, member 6)
Background This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]
Function Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.

The FasL intracellular domain (FasL ICD) cytoplasmic form induces gene transcription inhibition. [UniProt]
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PTM The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells.

N-glycosylated (PubMed:9228058). Glycosylation enhances apoptotic activity (PubMed:27806260).

Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization.

Monoubiquitinated. [UniProt]
Title Download Link
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