ARG82459

Mouse IFNAR1 ELISA Kit

Mouse IFNAR1 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82459-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82459-002 Standard 2 X 10 ng/vial 4°C
ARG82459-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82459-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82459-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82459-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82459-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82459-008 25X Wash buffer 20 ml 4°C
ARG82459-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82459-010 STOP solution 10 ml (Ready to use) 4°C
ARG82459-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82459 Mouse IFNAR1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IFNAR1 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name IFNAR1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.3%
Inter-Assay CV: 5.8%
Alternate Names Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; IFRC; IFNAR; AVP; IFN-alpha/beta receptor 1; CRF2-1; IFN-R-1; Type I interferon receptor 1; IFNBR; Interferon alpha/beta receptor 1; IFN-alpha-REC

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 15975 Mouse IFNAR1

Swiss-port # P33896 Mouse Interferon alpha/beta receptor 1

Gene Symbol IFNAR1
Gene Full Name interferon (alpha, beta and omega) receptor 1
Background The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. [provided by RefSeq, Jul 2008]
Function Associates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves. Can also transduce IFNB signals without the help of IFNAR2, and not activating the Jak-STAT pathway. [UniProt]
Cellular Localization Isoform 1: Cell membrane; Single-pass type I membrane protein. Late endosome. Lysosome. Note=Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes. [UniProt]
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PTM Ubiquitinated, leading to its internalization and degradation (PubMed:14532120, PubMed:15337770). Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation (PubMed:18056411). The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex (PubMed:24075985).

Phosphorylated on serine residues in response to interferon binding; this promotes interaction with FBXW11 and ubiquitination (PubMed:14532120, PubMed:15337770, PubMed:24075985). Phosphorylated on tyrosine residues by TYK2 tyrosine kinase (PubMed:7526154). Phosphorylated on tyrosine residues in response to interferon (PubMed:10049744).

Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2. [UniProt]
Title Download Link
ARG82459 Mouse IFNAR1 ELISA Kit User manual download icon Download