ARG82349

Mouse MPL / TPOR ELISA Kit

Mouse MPL / TPOR ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82349-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82349-002 Standard 2 X 10 ng/vial 4°C
ARG82349-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82349-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82349-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82349-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82349-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82349-008 25X Wash buffer 20 ml 4°C
ARG82349-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82349-010 STOP solution 10 ml (Ready to use) 4°C
ARG82349-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82349 Mouse MPL / TPOR ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse MPL / TPOR in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name MPL / TPOR
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.7%
Inter-Assay CV: 6.5%
Alternate Names Proto-oncogene c-Mpl; MPLV; CD110; Thrombopoietin receptor; C-MPL; TPO-R; Myeloproliferative leukemia protein; TPOR; THCYT2; CD antigen CD110

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17480 Mouse MPL

Swiss-port # Q08351 Mouse Thrombopoietin receptor

Gene Symbol MPL
Gene Full Name MPL proto-oncogene, thrombopoietin receptor
Background In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq, Jul 2008]
Function Receptor for thrombopoietin. May represent a regulatory molecule specific for TPO-R-dependent immune responses. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Golgi apparatus. Cell surface. [UniProt]
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PTM Ubiquitination at Lys-553 and Lys-573 targets MPL for degradation by both the lysosomal and proteasomal pathways. The E3 ubiquitin-protein ligase CBL significantly contributes to this ubiquitination. [UniProt]
Title Download Link
ARG82349 Mouse MPL-TPOR ELISA kit User manual download icon Download