ARG81752
Mouse OLR1 / LOX1 ELISA Kit
Mouse OLR1 / LOX1 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81752-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81752-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81752-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81752-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81752-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81752-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81752-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81752-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81752-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81752-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81752-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81752 Mouse OLR1 / LOX1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse OLR1 / LOX1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | OLR1 / LOX1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.1%; Inter-Assay CV: 7.2% |
| Alternate Names | Lectin-like oxidized LDL receptor 1; Lectin-type oxidized LDL receptor 1; LOX-1; CLEC8A; SLOX1; LOXIN; hLOX-1; Oxidized low-density lipoprotein receptor 1; Lectin-like oxLDL receptor 1; C-type lectin domain family 8 member A; Ox-LDL receptor 1; LOX1; SCARE1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9EQ09 Mouse Oxidized low-density lipoprotein receptor 1 |
|---|---|
| Gene Symbol | OLR1 |
| Gene Full Name | oxidized low density lipoprotein (lectin-like) receptor 1 |
| Background | This gene encodes a low density lipoprotein receptor that belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010] |
| Function | Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria. [UniProt] |
| Highlight | Related products: OLR1 antibodies; OLR1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The intrachain disulfide-bonds prevent N-glycosylation at some sites. N-glycosylated. [UniProt] |
| Title | Download Link |
|---|---|
| ARG81752 Mouse OLR1 / LOX1 ELISA Kit User's manual |
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