ARG81667

Mouse Osteoprotegerin ELISA Kit

Mouse Osteoprotegerin ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81667-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81667-002 Standard 2 X 10 ng/vial 4°C
ARG81667-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81667-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81667-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81667-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81667-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81667-008 25X Wash buffer 20 ml 4°C
ARG81667-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81667-010 STOP solution 10 ml (Ready to use) 4°C
ARG81667-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81667 Mouse Osteoprotegerin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Osteoprotegerin in serum, plasma (EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Osteoprotegerin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 46.85 pg/ml
Sample Type Serum, plasma (EDTA) and cell culture supernatants.
Standard Range 93.7 - 6000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.5%; Inter-Assay CV: 6.0%
Alternate Names OCIF; Osteoclastogenesis inhibitory factor; Tumor necrosis factor receptor superfamily member 11B; PDB5; Osteoprotegerin; OPG; TR1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18383 Mouse TNFRSF11B

Swiss-port # O08712 Mouse Tumor necrosis factor receptor superfamily member 11B

Gene Symbol TNFRSF11B
Gene Full Name tumor necrosis factor receptor superfamily, member 11b
Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]
Function Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis. [UniProt]
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PTM N-glycosylated. Contains sialic acid residues.

The N-terminus is blocked. [UniProt]
Title Download Link
ARG81667 Mouse Osteoprotegerin ELISA Kit User's manual download icon Download