ARG82084
Mouse TLR2 ELISA Kit
Mouse TLR2 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82084-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82084-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82084-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82084-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82084-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82084-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82084-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82084-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82084-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82084-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82084-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82084 Mouse TLR2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse TLR2 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | TLR2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3%; Inter-Assay CV: 7.0% |
| Alternate Names | CD antigen CD282; TIL4; CD282; Toll/interleukin-1 receptor-like protein 4; Toll-like receptor 2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TLR2 |
| Gene Full Name | toll-like receptor 2 |
| Background | The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. [provided by RefSeq, Jul 2008] |
| Function | Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, phagosome membrane; Single-pass type I membrane protein. Membrane raft. Note=Does not reside in lipid rafts before stimulation but accumulates increasingly in the raft upon the presence of the microbial ligand. In response to diacylated lipoproteins, TLR2:TLR6 heterodimers are recruited in lipid rafts, this recruitment determines the intracellular targeting to the Golgi apparatus. [UniProt] |
| Highlight | Related products: TLR2 antibodies; TLR2 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2. [UniProt] |
| Title | Download Link |
|---|---|
| ARG82084 Mouse TLR2 ELISA Kit User's manual |
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