ARG82346

Mouse VEGFB ELISA Kit

Mouse VEGFB ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82346-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82346-002 Standard 2 X 10 ng/vial 4°C
ARG82346-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82346-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82346-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82346-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82346-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82346-008 25X Wash buffer 20 ml 4°C
ARG82346-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82346-010 STOP solution 10 ml (Ready to use) 4°C
ARG82346-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82346 Mouse VEGFB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse VEGFB in serum, plasma (heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name VEGFB
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.8%
Inter-Assay CV: 7.0%
Alternate Names VEGF-related factor; VEGFL; VEGF-B; VRF; Vascular endothelial growth factor B

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22340 Mouse VEGFB

Swiss-port # P49766 Mouse Vascular endothelial growth factor B

Gene Symbol VEGFB
Gene Full Name vascular endothelial growth factor B
Background This gene encodes a member of the PDGF (platelet-derived growth factor)/VEGF (vascular endothelial growth factor) family. The VEGF family members regulate the formation of blood vessels and are involved in endothelial cell physiology. This member is a ligand for VEGFR-1 (vascular endothelial growth factor receptor 1) and NRP-1 (neuropilin-1). Studies in mice showed that this gene was co-expressed with nuclear-encoded mitochondrial genes and the encoded protein specifically controlled endothelial uptake of fatty acids. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Sep 2011]
Function Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. [UniProt]
Cellular Localization Secreted. Note=Secreted but remains associated to cells or to the extracellular matrix unless released by heparin. [UniProt]
Highlight Related products:
VEGF antibodies; VEGF ELISA Kits; VEGF Duos / Panels; VEGF recombinant proteins;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM VEGF-B186 is O-glycosylated. [UniProt]
Title Download Link
ARG82346 Mouse VEGFB ELISA Kit User manual download icon Download