ARG81164
Phosphorylated Neurofilament NF-H ELISA Kit
Phosphorylated Neurofilament NF-H ELISA Kit for ELISA and Chicken,Cow,Horse,Human,Mouse,Pig,Rat
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Overview
| Product Description | ARG81164 Phosphorylated Neurofilament NF-H ELISA Kit is an Enzyme Immunoassay kit for the quantification of Phosphorylated Neurofilament NF-H in plasma, serum, CSF and tissue extracts. |
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| Tested Reactivity | Hu, Ms, Rat, Chk, Cow, Hrs, Pig |
| Tested Application | ELISA |
| Target Name | Neurofilament NF-H |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sample Type | Plasma, serum, CSF and tissue extracts. |
| Standard Range | 0.156 - 10 ng/ml |
| Sample Volume | 50 µl |
| Precision | Intra-Assay CV: < 6%; Inter-Assay CV: < 10% |
| Alternate Names | Neurofilament heavy polypeptide; 200 kDa neurofilament protein; NF-H; Neurofilament triplet H protein; NFH |
Application Instructions
| Assay Time | ~ 3 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store components at 4°C or -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | NEFH |
| Gene Full Name | neurofilament, heavy polypeptide |
| Background | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008] |
| Function | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. [UniProt] |
| Highlight | Related products: Neurofilament antibodies; Neurofilament ELISA Kits; Neurofilament Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Calculated MW | 110 kDa |
| PTM | There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. |
Specific References
Therapeutic Effects of Combination of Nebivolol and Donepezil: Targeting Multifactorial Mechanisms in ALS
ELISA / Mouse / Serum
| Title | Download Link |
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| ARG81164 Phosphorylated Neurofilament NF-H ELISA Kit User's manual |
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