ARG81782

Rat CD62L / L-Selectin ELISA Kit

Rat CD62L / L-Selectin ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG81782-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81782-002 Standard 2 X 40 ng/vial 4°C
ARG81782-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81782-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81782-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81782-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81782-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81782-008 25X Wash buffer 20 ml 4°C
ARG81782-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81782-010 STOP solution 10 ml (Ready to use) 4°C
ARG81782-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81782 Rat CD62L / L-Selectin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat soluble CD62L / L-Selectin in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD62L / L-Selectin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 312.5 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 625 - 40000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.3%; Inter-Assay CV: 6.0%
Alternate Names Leukocyte surface antigen Leu-8; Leukocyte adhesion molecule 1; CD antigen CD62L; PLNHR; LSEL; CD62L; Leukocyte-endothelial cell adhesion molecule 1; L-selectin; LAM1; LNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; Lymph node homing receptor; LYAM1; LECAM1; LEU8; LAM-1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol SELL
Gene Full Name selectin L
Background This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]
Function Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. [UniProt]
Highlight Related products:
CD62L antibodies; CD62L ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Title Download Link
ARG81782 Rat sCD62L / sL-Selectin ELISA Kit User's manual download icon Download