ARG80236

Rat CXCL1 / CINC1 ELISA Kit

Rat CXCL1 / CINC1 ELISA Kit for ELISA and Rat

Immune System kit; Signaling Transduction kit
publication_link Publication1

Component

Cat No Component Name Package Temp
ARG80236-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80236-002 Standard (Lyophilized) 3 X 2 ng/vial 4°C
ARG80236-003 Standard diluent buffer 20 ml 4°C
ARG80236-004 Antibody conjugate concentrate 400 µl 4°C
ARG80236-005 Antibody diluent buffer 16 ml 4°C
ARG80236-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80236-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80236-008 20X Wash buffer 50 ml 4°C
ARG80236-009 TMB substrate 12ml 4°C (Protect from light)
ARG80236-010 STOP solution 12ml 4°C
ARG80236-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80236 Rat CXCL1 / CINC1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CXCL1 / CINC1 in serum, plasma and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human GRO alpha, GRO beta, GRO gamma;mouse MIP-2;rat CINC-2 alpha, CINC-2 beta, CINC-3
Target Name CXCL1 / CINC1
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 2 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 3.9 - 250 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names Growth-regulated alpha protein; SCYB1; Melanoma growth stimulatory activity; MGSA-a; GRO-alpha; GROa; NAP-3; FSP; 5-73; C-X-C motif chemokine 1; Neutrophil-activating protein 3; 6-73; 1-73; GRO1; MGSA; Platelet-derived growth factor-inducible protein KC; IL8 homologues

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 81503 Rat CXCL1

Swiss-port # P14095 Rat Growth-regulated alpha protein

Gene Symbol Cxcl1
Gene Full Name chemokine (C-X-C motif) ligand 1
Background This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Sep 2014]
Highlight Related products:
CXCL1 antibodies; CXCL1 ELISA Kits;
Related news:
Immune signaling protein TLR4 has opposing roles in breast cancer development
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Immune System kit; Signaling Transduction kit
PTM N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

Specific References

The effects of simulated childbirth trauma on the gene expression of neurotransmitter receptors in the bladder of female rats.

ELISA / Rat / Serum

Cheng-Yu Long et al.
J Formos Med Assoc.,  (2021)

publication_link

 

hr_line
Title Download Link
ARG80236 Rat CXCL1 / CINC1 ELISA Kit User's manual download icon Download