ARG80242

Rat VEGF ELISA Kit

Rat VEGF ELISA Kit for ELISA and Rat

Cancer kit; Cell Biology and Cellular Response kit; Metabolism kit; Signaling Transduction kit

Component

Cat No Component Name Package Temp
ARG80242-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80242-002 Standard (Lyophilized) 3 X 2 ng/vial 4°C
ARG80242-003 Standard diluent buffer 20 ml 4°C
ARG80242-004 Antibody conjugate concentrate 400 µl 4°C
ARG80242-005 Antibody diluent buffer 16 ml 4°C
ARG80242-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80242-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80242-008 20X Wash buffer 50 ml 4°C
ARG80242-009 TMB substrate 12 ml 4°C (Protect from light)
ARG80242-010 STOP solution 12 ml 4°C
ARG80242-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80242 Rat VEGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat VEGF in serum, plasma and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Mouse VEGF-B186
Target Name VEGF
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 15.6 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 31.25 - 2000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names MVCD1; Vascular permeability factor; VEGF-A; VPF; VEGF; Vascular endothelial growth factor A

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 83785 Rat VEGFA

Swiss-port # P16612 Rat Vascular endothelial growth factor A

Gene Symbol Vegfa
Gene Full Name vascular endothelial growth factor A
Background This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. [provided by RefSeq, Jul 2008]
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Research Area Cancer kit; Cell Biology and Cellular Response kit; Metabolism kit; Signaling Transduction kit
Title Download Link
ARG80242 Rat VEGF ELISA Kit User's manual download icon Download