ARG81958

Rat sRANK Ligand / TNFSF11 / TRANCE ELISA Kit

Rat sRANK Ligand / TNFSF11 / TRANCE ELISA Kit for ELISA and Rat

publication_link Publication1

Component

Cat No Component Name Package Temp
ARG81958-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81958-002 Standard 2 X 10 ng/vial 4°C
ARG81958-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81958-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81958-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81958-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81958-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81958-008 25X Wash buffer 20 ml 4°C
ARG81958-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81958-010 STOP solution 10 ml (Ready to use) 4°C
ARG81958-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81958 Rat sRANK Ligand / TNFSF11 / TRANCE ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat sRANK Ligand / TNFSF11 / TRANCE in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name sRANK Ligand / TNFSF11 / TRANCE
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.5%; Inter-Assay CV: 7.2%
Alternate Names TRANCE; Osteoprotegerin ligand; CD254; sOdf; Receptor activator of nuclear factor kappa-B ligand; OPTB2; RANKL; OPGL; Tumor necrosis factor ligand superfamily member 11; hRANKL2; TNF-related activation-induced cytokine; Osteoclast differentiation factor; ODF; CD antigen CD254

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 117516 Rat TNFSF11

Swiss-port # Q9ESE2 Rat Tumor necrosis factor ligand superfamily member 11

Gene Symbol TNFSF11
Gene Full Name tumor necrosis factor (ligand) superfamily, member 11
Background This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]
Function Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. [UniProt]
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PTM The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17. [UniProt]

Specific References

Ac-SDKP Attenuates Activation of Lung Macrophages and Bone Osteoclasts in Rats Exposed to Silica by Inhibition of TLR4 and RANKL Signaling Pathways.

ELISA / Rat / Serum

Fuyu Jin et al.
J Inflamm Res.,  (2021)

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