ARG65550

anti-CD3 antibody [UCHT1]

anti-CD3 antibody [UCHT1] for CyTOF®-candidate,Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Human,Primates

Cancer antibody; Developmental Biology antibody; Immune System antibody

Overview

Product Description

Mouse Monoclonal antibody [UCHT1] recognizes CD3

Tested Reactivity Hu, NHuPrm
Tested Application CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IP
Specificity The clone UCHT1 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. The UCHT1 antibody reacts with the epsilon chain of the CD3 complex.
HLDA I; WS Code T 3
HLDA III; WS Code T 126
HLDA III; WS Code T 471
HLDA VI; WS Code T 6T-CD3.1
Host Mouse
Clonality Monoclonal
Clone UCHT1
Isotype IgG1
Target Name CD3
Antigen Species Human
Immunogen human thymocytes followed by Sezary T cells
Conjugation Un-conjugated
Alternate Names T-cell receptor T3 delta chain; T3D; IMD19; CD3-DELTA; CD antigen CD3d; T-cell surface glycoprotein CD3 delta chain

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 4 µg/ml
FuncStAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
Application Note FACS and IHC-Fr: The epitope for UCHT1, CD3 is resistant to fixation. For purposes where pre-fixed cells are stained, this antibody is recommended.
Functional studies: The immobilized UCHT1 antibody initiates a signaling pathway resulting in T cell activation and proliferation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 915 Human CD3D

Swiss-port # P04234 Human T-cell surface glycoprotein CD3 delta chain

Gene Symbol CD3D
Gene Full Name CD3d molecule, delta (CD3-TCR complex)
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Function The CD3 complex mediates signal transduction. [UniProt]
Highlight Related products:
CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; CD3 recombinant proteins; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
New antibody panels and duos for Tumor immune microenvironment
Tumor-Infiltrating Lymphocytes (TILs)
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Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody
Calculated MW 19 kDa

Clone References

Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies.

Siegers GM et al.
J Exp Med.,  (2007)

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Expansion of regulatory T cells in patients with Langerhans cell histiocytosis.

Senechal B et al.
PLoS Med.,  (2007)

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Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency.

Rieux-Laucat F et al.
N Engl J Med.,  (2006)

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Identification and characterization of human pulmonary dendritic cells.

Demedts IK et al.
Am J Respir Cell Mol Biol.,  (2005)

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