ARG66888

anti-Caspase 3 (cleaved) antibody

Publication2

Ordering Information

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Price (USD)

100 μg

355.00

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Datasheet
Publication (2)

Overview

Product Description	Mouse Monoclonal antibody recognizes Caspase 3 (cleaved)
Tested Reactivity	Hu, Ms, Rat
Tested Application	ICC/IF, IHC-P, WB
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Target Name	Caspase 3 (cleaved)
Antigen Species	Human
Immunogen	Recombinant protein of cleaved Caspase 3.
Conjugation	Un-conjugated
Alternate Names	CPP-32; Caspase-3; EC 3.4.22.56; Apopain; CASP-3; CPP32; Cysteine protease CPP32; SCA-1; SREBP cleavage activity 1; CPP32B; Protein Yama

Application Instructions

Application Suggestion

Tested Application	Dilution
ICC/IF	1:50 - 1:200
IHC-P	1:100 - 1:200
WB	1:250 - 1:1000

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control

HeLa, NIH/3T3 and Rat brain

Observed Size

~ 20 kDa (cleaved form)

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS (pH 7.4), 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol and 0.5% BSA
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 12367 Mouse CASP3 GeneID: 25402 Rat CASP3 GeneID: 836 Human CASP3 Swiss-port # P42574 Human Caspase-3 Swiss-port # P55213 Rat Caspase-3 Swiss-port # P70677 Mouse Caspase-3 More
Gene Symbol	CASP3
Gene Full Name	caspase 3, apoptosis-related cysteine peptidase
Background	The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]
Function	Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-\|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. [UniProt]
Cellular Localization	Cytoplasm. [UniProt]
Calculated MW	32 kDa
PTM	Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. [UniProt]