ARG59047

anti-NUCB2 (Nesfatin 1) antibody

anti-NUCB2 (Nesfatin 1) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NUCB2 (Nesfatin 1)
Tested Reactivity Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NUCB2 (Nesfatin 1)
Antigen Species Mouse
Immunogen Recombinant protein corresponding to V25-L106 of Mouse NUCB2.
Conjugation Un-conjugated
Alternate Names HEL-S-109; NEFA; Nucleobindin-2; Gastric cancer antigen Zg4; Prepronesfatin; DNA-binding protein NEFA

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P0.5 - 1 µg/ml
WB0.1 - 0.5 µg/ml
Application Note IHC-P: Antigen Retrieval: By heat mediation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 53322 Mouse NUCB2

GeneID: 59295 Rat NUCB2

Swiss-port # P81117 Mouse Nucleobindin-2

Swiss-port # Q9JI85 Rat Nucleobindin-2

Gene Symbol NUCB2
Gene Full Name nucleobindin 2
Background This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]
Function Calcium-binding protein. May have a role in calcium homeostasis.

Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance (By similarity). [UniProt]
Cellular Localization Cytoplasm. Perikaryon. Endoplasmic reticulum. Golgi apparatus. Nucleus envelope. Membrane; Peripheral membrane protein. Secreted. In dendrites and perikarya of brain neurons. Abundant in the ER cisternae and nuclear envelope, but not detected in Golgi, mitochondria or nucleoplasm in neurons. In cell culture, cytoplasmic and secreted. [UniProt]
Calculated MW 50 kDa