ARG80157

Human IGFBP1 ELISA Kit

Human IGFBP1 ELISA Kit for ELISA and Human

Cell Biology and Cellular Response kit; Signaling Transduction kit

Component

Cat No Component Name Package Temp
ARG80157-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80157-002 Standard (Lyophilized) 5 X 0.75 ng/vial 4°C
ARG80157-003 Standard diluent buffer 20 ml 4°C
ARG80157-004 Antibody conjugate concentrate 400 µl 4°C
ARG80157-005 Antibody diluent buffer 16 ml 4°C
ARG80157-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80157-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80157-008 20X Wash buffer 50 ml 4°C
ARG80157-009 TMB substrate 12ml 4°C (Protect from light)
ARG80157-010 STOP solution 12ml 4°C
ARG80157-011 Plate sealer 4 strips Room temperature

Overview

Product Description

ARG80157 Human IGFBP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IGFBP1 in Serum, Plasma, Cell culture supernatants.

Tested Reactivity Hu
Tested Application ELISA
Target Name IGFBP1
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 25 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 46.8 - 3000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names IBP-1; IBP1; PP12; IGF-BP25; Insulin-like growth factor-binding protein 1; hIGFBP-1; IGFBP-1; Placental protein 12; AFBP; IGF-binding protein 1

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IGFBP1
Gene Full Name insulin-like growth factor binding protein 1
Background This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]
Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. [UniProt]
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Research Area Cell Biology and Cellular Response kit; Signaling Transduction kit
PTM Phosphorylated; probably by casein kinase II. Phosphorylation alters the affinity of the protein for IGFs. In amniotic fluid, the unmodified protein is the most abundant form, while mono-, bi-, tri- and tetraphosphorylated forms are present in decreasing amounts. The phosphorylation state may influence the propensity to proteolysis.
Title Download Link
ARG80157 Human IGFBP1 ELISA Kit User's manual download icon Download