ARG80215
Mouse IgG (Total) ELISA Kit
Mouse IgG (Total) ELISA Kit for ELISA and Mouse
Immune System kit
Publication1
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80215-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80215-002 | Standard (Lyophilized) | 3 X 10 ng/vial | 4°C |
| ARG80215-003 | Standard diluent buffer | 40 ml | 4°C |
| ARG80215-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80215-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80215-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80215-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80215-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80215-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80215-010 | STOP solution | 12ml | 4°C |
| ARG80215-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80215 Mouse IgG (Total) ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IgG (Total) in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | This ELISA kit might cross-react with IgG from other species. |
| Target Name | IgG (Total) |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 0.08 ng/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 0.156 - 10 ng/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | Immunoglobulin G |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Antibodies are major components of the immune system. IgG is the main antibody isotype found in blood and extracellular fluid allowing it to control infection of body tissues. By binding many kinds of pathogens—representing viruses, bacteria, and fungi—IgG protects the body from infection. It does this via several immune mechanisms: IgG-mediated binding of pathogens causes their immobilization and binding together via agglutination; IgG coating of pathogen surfaces (known as opsonization) allows their recognition and ingestion by phagocytic immune cells; IgG activates the classical pathway of the complement system, a cascade of immune protein production that results in pathogen elimination; IgG also binds and neutralizes toxins. IgG also plays an important role in antibody-dependent cell-mediated cytotoxicity (ADCC) and intracellular antibody-mediated proteolysis, in which it binds to TRIM21 (the receptor with greatest affinity to IgG in humans) in order to direct marked virions to the proteasome in the cytosol. IgG is also associated with Type II and Type III Hypersensitivity. IgG antibodies are generated following class switching and maturation of the antibody response and thus participate predominantly in the secondary immune response. IgG is secreted as a monomer that is small in size allowing it to easily perfuse tissues. It is the only isotype that has receptors to facilitate passage through the human placenta, thereby providing protection to the fetus in utero. Along with IgA secreted in the breast milk, residual IgG absorbed through the placenta provides the neonate with humoral immunity before its own immune system develops. Colostrum contains a high percentage of IgG, especially bovine colostrum. In individuals with prior immunity to a pathogen, IgG appears about 24–48 hours after antigenic stimulation. |
|---|---|
| Highlight | Related products: IgG antibodies; IgG ELISA Kits; IgG Duos / Panels; IgG recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Immune System kit |
Specific References
Follicular regulatory T cells are associated with β-cell autoimmunity and the development of type 1 diabetes.
ELISA / Mouse
| Title | Download Link |
|---|---|
| ARG80215 Mouse IgG (Total) ELISA Kit User's manual |
 Download
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