ARG80215

Mouse IgG (Total) ELISA Kit

Mouse IgG (Total) ELISA Kit for ELISA and Mouse

Immune System kit
publication_link Publication1

Component

Cat No Component Name Package Temp
ARG80215-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80215-002 Standard (Lyophilized) 3 X 10 ng/vial 4°C
ARG80215-003 Standard diluent buffer 40 ml 4°C
ARG80215-004 Antibody conjugate concentrate 400 µl 4°C
ARG80215-005 Antibody diluent buffer 16 ml 4°C
ARG80215-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80215-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80215-008 20X Wash buffer 50 ml 4°C
ARG80215-009 TMB substrate 12ml 4°C (Protect from light)
ARG80215-010 STOP solution 12ml 4°C
ARG80215-011 Plate sealer 4 strips Room temperature

Overview

Product Description

ARG80215 Mouse IgG (Total) ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IgG (Total) in Serum, Plasma, Cell culture supernatants.

Tested Reactivity Ms
Tested Application ELISA
Specificity This ELISA kit might cross-react with IgG from other species.
Target Name IgG (Total)
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 0.08 ng/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 0.156 - 10 ng/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names Immunoglobulin G

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background Antibodies are major components of the immune system. IgG is the main antibody isotype found in blood and extracellular fluid allowing it to control infection of body tissues. By binding many kinds of pathogens—representing viruses, bacteria, and fungi—IgG protects the body from infection. It does this via several immune mechanisms: IgG-mediated binding of pathogens causes their immobilization and binding together via agglutination; IgG coating of pathogen surfaces (known as opsonization) allows their recognition and ingestion by phagocytic immune cells; IgG activates the classical pathway of the complement system, a cascade of immune protein production that results in pathogen elimination; IgG also binds and neutralizes toxins. IgG also plays an important role in antibody-dependent cell-mediated cytotoxicity (ADCC) and intracellular antibody-mediated proteolysis, in which it binds to TRIM21 (the receptor with greatest affinity to IgG in humans) in order to direct marked virions to the proteasome in the cytosol. IgG is also associated with Type II and Type III Hypersensitivity. IgG antibodies are generated following class switching and maturation of the antibody response and thus participate predominantly in the secondary immune response. IgG is secreted as a monomer that is small in size allowing it to easily perfuse tissues. It is the only isotype that has receptors to facilitate passage through the human placenta, thereby providing protection to the fetus in utero. Along with IgA secreted in the breast milk, residual IgG absorbed through the placenta provides the neonate with humoral immunity before its own immune system develops. Colostrum contains a high percentage of IgG, especially bovine colostrum. In individuals with prior immunity to a pathogen, IgG appears about 24–48 hours after antigenic stimulation.
Highlight Related products:
IgG antibodies; IgG ELISA Kits; IgG Duos / Panels; IgG recombinant proteins;
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Simplify the ELISA analysis by GainData
Research Area Immune System kit

Specific References

Follicular regulatory T cells are associated with β-cell autoimmunity and the development of type 1 diabetes.

ELISA / Mouse

Xu Xinyu et al.
J Clin Endocrinol Metab.,  (2019)

publication_link

 

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